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eIF3E (V116) pAb

eIF3E (V116) pAb

Cat no: BS3296


Supplier: Bioworld Technology, Inc.
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Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFE, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.
Catalogue number: BS3296
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: O00303
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 91-140 of Human eIF3E.
Species: eIF3E (V116) pAb detects endogenous levels of eIF3E protein.
Molecular weight: ~ 38 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: EIF3S5; EIF3F; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; EC=3.4.19.12; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47
Additional info: For research use only, not for use in diagnostic procedure.

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