Elafin (Elastase-specific Inhibitor, ESI, Peptidase Inhibitor 3, PI-3, Protease Inhibitor WAP3, Skin-derived Antileukoproteinase, SKALP, WAP Four-disulfide Core Domain Protein 14, PI3, WAP3, WFDC14)

Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100\nImmunohistochemistry: Paraffin\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2225-02

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Alkemade, J et al; Demonstration of skin-derived antileukoproteinase (SKALP) and its target enzyme human leukocyte elastase in squamous carcinoma. J Pathol 1994, 174: 121. 2. Bergen van, H et al; Expression of SKALP/elafin during wound healing in human skin. Arch Dermatol Res 1996, 288: 458. 3. Pfundt, R et al; Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia. J Clin Invest 1996, 98: 1389. 4. Schalkwijk, J et al; Localization of SKALP/elafin. J Invest Dermatol 1993, 100: 390. 5. Schalkwijk, J et al; The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a c-terminal four-disulphide core. Biochem J 1999, 340: 569. 6. Wiedow, O et al; Elafin: an elastase-specific inhibitor of human skin. J Biol Chem 1990, 265: 14791.

Additional Info

Recognizes human Elafin.