ENaC(delta) (P446) pAb

The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that is located on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands and other organs. ENaC is formed by heteromultimerization of four homologous subunits, (alpha), (beta), (gamma) and (delta). The most frequently formed heterotetramer consists of 2(alpha), 1(beta), and 1(gamma) subunit, but the (alpha) subunit can be replaced by a (delta) subunit. The (alpha)ENaC gene maps to human chromosome 12p13, and expresses a glycosylated protein. Both the (beta) and (gamma)ENaC genes map to human chromosome 16p12, and the (gamma)ENaC transcript is detected as a glycosylated protein. The carboxy-terminus of all ENaC subunits contains PY motifs, which interact with the ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-function mutations involving the PY motif cause Liddle's syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption. Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension.

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SPECIFICATIONS

Catalog Number

BS3070

Size

100ug/100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 430-480 of Human ENaC(delta).

Species

ENaC(delta) (P446) pAb detects endogenous levels of ENaC(delta) protein.

Dilutions

WB: 1:500~1:1000\nIF: 1:50~1:200

Swiss Prot

P51172

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul