Eotaxin 2 (MPIF-2,Myeloid progenitor inhibitory factor-2, Chemokine-beta-6, Ck-beta-6, SCYA24, small inducible cytokine A24, CCL24)

This protein of 78 amino acids belongs to the group of cytokines known as CC-Chemokines. Eotaxin-2 is identical with MPIF-2 (Myeloid progenitor inhibitory factor-2) and is known also as Chemokine-beta-6 (abbr. Ck-beta-6). The new designation is SCYA24 [small inducible cytokine A24]. The factor has been renamed CCL24. See also: SCY family of cytokines for a systematic nomenclature. The eotaxin-2 and eotaxin-3 genes are located within a region of approximately 40 kilobases on human chromosome 7q11.23.\n\nThree variants of 73, 75, and 76 residues truncated at the C-terminus have been described also. The protein is distantly related to, but functionally very similar, to Eotaxin. Murine eotaxin-2 appears to be produced constitutively in the jejunum and spleen. Production is induced by allergen challenge and IL4 overexpression (Zimmermann et al, 2000).\n\nEotaxin-2 induces chemotaxis of eosinophils and basophils, and the release of histamine and leukotriene C-4 from basophils primed with IL3. Eotaxin-2 activates chemotaxis-related events such as actin polymerization, and the respiratory burst almost as efficiently as Eotaxin. Monoclonal antibodies (7B11) selectively blocking the eotaxin receptor, CCR3, also block migration and release responses. Intradermal injection of eotaxin-2 in a rhesus monkey (100 or 1,000 pmol per site) induces a marked local infiltration of eosinophils.\n\nApplications:\nDirect ELISA\n\nRecommended Dilution:\nDirect ELISA: 1:10,000 dilution will yield 0.4 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig\n\nReconstitution:\nReconstitute with dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3351-07E

Size

500ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2.

P Type

Mab

Purity

Purified by ion exchange chromatography.

Isotype

IgG1

References

1. Miller JD, Pegelow DF, Dempsey JA, (2005) J Physiol Jan 13 2. Deheyn DD, Gendreau P, Latz MI, (2005) Mar Environ Res Jul;60(1):1-33. 3. Sosnowski M, Clark E, Macfarlane PW, (2005) Ann Noninvasive Electrocardiol Jan;10(1):7-15. 4. Zinkstok RT, Witte S, Eikema KS, (2005) Opt Lett Jan 1;30(1):78-80. 5. Schlatter A, Krainer L, Paschotta R, (2005) Opt Lett Jan 1;30(1):44-6. 6. Fernandes FC, van Spaendonck RL, (2005) IEEE Trans Image Process Jan;14(1):110-24.

Additional Info

Recognizes human Eotaxin-2.