Estrogen Receptor-a (ER, ER-alpha, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, ESR1, ESR, NR3A1)

The estrogen receptor alpha (ERa), a transcription factor that controls the expression of a number of genes involved in cellular differentiation and proliferation in a wide variety of tissues, is regulated by ligand binding and phosphorylation. ER contains at least two transcriptionally active domains: constitutively active F-1 at the ER N-terminus of the protein and ligand-dependent AF-2 at the ER C-terminus. Although ligand binding is considered essential for the full activation of ER, it has long been recognized that the receptor is subject to post-translational alterations, such as phosphorylation, which also regulated its activity. ER phosphorylation patterns appear to be cell type-specific. Serine 167 has been shown to be phosphorylated by p90rsk1 in vitro and to regulate ER AF-1-dependent transcriptional activation in vivo.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-12A

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Rofatsky, I. et al. J Biol Chem 274, 22296 (1999). 2. Joel, P. et al. Mol Cell Biol 18, 1978 (1998). 3. Blobel, G. et al. Mol Cell Biol 16, 1687 (1996). 4. McInerney, E. et al. Proc Natl Acad Sci USA 93, 10069 (1996). 5. Kraus, W. et al. Proc Natl Acad Sci USA 92, 12314 (1995). 6. Gaub, M. et al. Cell 63, 1267 (1990).

Additional Info

Recognizes human ER alpha. Species Crossreactivity: mouse and rat.