Home  >  Products  >  F1Aa (F1A alpha, F1A-alpha, FEM1 beta, FEM1-beta, FEM1b, Fem1 Homolog B, FEM1-like Death Receptor Binding Protein, DKFZP451E0710, KIAA0396)
F1Aa (F1A alpha, F1A-alpha, FEM1 beta, FEM1-beta, FEM1b, Fem1 Homolog B, FEM1-like Death Receptor Binding Protein, DKFZP451E0710, KIAA0396)

F1Aa (F1A alpha, F1A-alpha, FEM1 beta, FEM1-beta, FEM1b, Fem1 Homolog B, FEM1-like Death Receptor Binding Protein, DKFZP451E0710, KIAA0396)

Cat no: F0001-05E

Supplier: United States Biological
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Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1beta (1,2). F1Aalpha/FEM1beta is the homologue of C. elegans sex determining protein FEM-1 (1,2). FEM-1/F1Aalpha is cleaved by CED-3 and caspase (3). FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1 (3). Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues (1,2) Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml, Mouse liver tissue lysate can be used as positive control and an approximately 70kD band can be detected. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0001-05E
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified immunoglobulin
References: 1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8. 2. Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20 3. Chan SL, Yee KS, Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275(24):17925-8.
Additional info: Recognizes human F1Aalpha. Species Crossreactivity: Mouse and rat.

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