F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC)

Mouse F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. \n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50, Fix with 1% paraformaldehyde (3).\nWestern Blot: 1:50, Mouse bone-marrow derived macrophages; non-reduced. Detects a band at ~125kD (3). Reduction with 2-mercaptoethanol destroys the antigen.\nImmunohistochemistry (frozen): 1:50, Tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT. Incubation with 0.02M sodium azide in PBS, 0.1% H2O2 for 10min at RT to destroy endogenous peroxidase. Use spleen as positive control \nImmunohistochemistry (paraffin): 1:50, Fixation in 10% neutral buffered formalin for 24 hours; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer. Use splenic macrophages as positive control (5).\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Mouse macrophages\n\nNegative Control: Mouse fibroblasts or granulocytes\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F0001-51B

Size

100ug

Applications

FC, IHC, WB

Hosts

Rat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Stevceva, L., et al., BMC Clin. Pathol. 1: 3 (2001). 2. Schaller, E., et al., Mol. Cell Biol. 22: 8035 (2002). 3. Malorny, U., et al., Cell Tissue Res. 243: 421 (1986). 4. Leenen, P., et al., J. Immunol. Methods 174: 5 (1994). 5. Mackler, A., et al., Biol. Reprod. 61: 879 (1999).

Additional Info

Recognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. F0001-51B is a macrophage marker capable of distinguishing non-destructive from destructive inflammation processes in the pancreas. It is also a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Species Crossreactivity: human (heart macrophages). Does not crossreact with mouse granulocytes, mast cells, platelets, lymphocytes, fibroblasts, or endothelial cells.