Factor IX is one of the key factors for blood coagulation. It can be transformed into two-chained activated form (FactorIXa) by factors in both coagulation pathways: by Factor XIa of the contact pathway or by Factor VIIa of the tissue factor pathway. In the presence of Factor VII cofactor, Ca++ and membrane phospholipids, Factor IXa activates Factor X. The activated Factor X (Factor Xa) triggers the transformation of prothrombin to thrombin, thus results in blood coagulation. Hemophilia B is a disease caused by Factor IX deficiency.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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