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Factor VIII, A2 Domain

Factor VIII, A2 Domain

Cat no: F0016-10E

Supplier: United States Biological
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Hemophilia A is an X-linked, recessive, bleeding disorder caused by a deficienty in the activity of coagulation Factor VIII. Affected individuals develop a variable phenotype of hemorrhage into joints and muscles, easy bruising, and prolonged bleeding from wounds. The disorder is caused by heterogeneous mutations in the Factor VIII gene. Up to 30% of hemophilia A patients given therapeutic Factor VIII can make inhibitory antibodies, the majority of which are reactive with its C2 and A2 domains Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0016-10E
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 500ug
Form: Supplied as lyophilized from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.4, 0.01% sodium azide. Reconstitute with 500ul ddH2O.
P type: Mab
Isotype: IgG1
Purity: Gel filtration and anion exchange chromatograph.(same/more than)98% pure (SDS-PAGE).
Additional info: Recognizes human factor VIII and human factor VIIIa in solid-phase ELISA and human factor VIII A2 domain in immunoblotting techniques.

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