FAF1 (UBX domain-containing protein 12, UBX domain-containing protein 3A)

Fas-associated factor-1 (FAF1) is a Fas-binding pro-apoptotic protein that is a component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 is involved in negative regulation of NF-kappaB activation. In addition to the previously known function as a component of the Fas death-inducing signaling complex, i.e. NF-kappaB activity suppressor by cytoplasmic retention of NF-kappaB p65 via physical interaction. hFAF1 was expressed abundantly in testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD protein in some cell lines.\n\nUniProt Number:\nQ9UNN5\n\nGene ID:\nFAF1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144505

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. J Biol Chem. 2004 Jan 23;279(4):2544-9. Epub 2003 Nov 4.Fas-associated factor-1 inhibits nuclear factor-kappaB (NF-kappaB) activity by interfering with nuclear translocation of the RelA (p65) subunit of NF-kappaB.Research Center for Biomedicinal Resources and the Division of Life Science, PaiChai University, Daejeon 302-735, Korea.\n2. 1\\Ryu, S. W., Chae, S. K., Lee, K. J., Kim, E. Identification and characterization of human Fas associated factor 1, hFAF1. Biochem. Biophys. Res. Commun. 262: 388-394, 1999.

Additional Info

Recognizes human, mouse and rat FAF1. No crossreactivity with other proteins.