FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor Subunit alpha, IgE Receptor)

High affinity Fc receptor for IgE (Fc epsilon R1) plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognises the extracellular D1 domain of the Fc epsilon R1 alpha chain.\nFc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher. \n\nRecommended Negative Control:\nI1904-84Q3: IgG2b Murine Negative Control\n\nHybridoma: \nSp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F0025-06B2

Size

100ug

Applications

ELISA, FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Vangelista, L. et al. (2002) A minimal receptor-Ig chimera of human Fc epsilon R1 alpha-chain efficiently binds secretory and membrane IgE. Protein Engineering. 15: 51-57, 2. Vangelista, L. et al. (2002) Efficient folding of the Fc epsilonR1 alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1. J. Mol. Biol. 322: 815-25

Additional Info

Recognizes human Human Fc Epsilon R1, alpha.

Alternative Names

Fc Fragment of IgE, High Affinity I, Receptor for alpha polypeptide; IgE Fc Receptor Subunit alpha; Fc-epsilon RI-alpha