FEN1 (A119) pAb

DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.

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SPECIFICATIONS

Catalog Number

BS1989

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 100-150 of Human FEN1.

Species

FEN-1 (A119) pAb detects endogenous levels of FEN-1 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

P39748

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 42 kDa

Alternative Names

FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; DNase IV; FEN1; Flap endonuclease. FEN-1. Flap exonuclease. FEN1. Five prime Exonuclease