FGF Receptor 1 (Fibroblast Growth Gactors) (Biotin)

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCy (4,5).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137434

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 2 mg/ml BSA, and 50% glycerol. Labeled with biotin.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. 2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. 3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. 4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. 5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

Additional Info

Recognizes endogenous levels of total human FGF receptor 1. Species Crossreactivity: mouse, rat, monkey. Does not cross-react with other FGF receptor family members.