FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)

FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32,000\nWestern Blot: 0.05-0.3ug/ml, observed in human muscle lysates on ~30kD bands\nImmunohistochemistry (Paraffin): 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4149-77J

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can, Prc

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Tang T, Lai NC, Hammond HK, Roth DM, Yang Y, Guo T, Gao MH. Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice. J Am Coll Cardiol. 2010 Apr 6;55(14):1476-86. 2. Schessl J, Taratuto AL, Sewry C, Battini R, Chin SS, Maiti B, Dubrovsky AL, Erro MG, Espada G, Robertella M, Saccoliti M, Olmos P, Bridges LR, Standring P, Hu Y, Zou Y, Swoboda KJ, Scavina M, Goebel HH, Mitchell CA, Flanigan KM, Muntoni F, B

Additional Info

Recognizes human FHL1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, porcine and rat.