FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30-32kD member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40-92; 101-153; 162-212; 221-275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53-279. Over aa 167-279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/mldetection limit for rhFHL2 is ~ 0.5ng/well
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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