FHOD1 (FH1/FH2 Domain-containing Protein 1, Formin Homolog Overexpressed in Spleen 1, FHOS, Formin Homology 2 Domain-containing Protein 1, FHOS, FHOS1)

FHOD1, a predominantly cytoplasmic protein, is a 1164aa containing member of formin:diaphanous family of proteins that contains a FH1 (formin homology 1) domain, a FH2 (formin homology 2) domain and a GBD/FH3 (Rho GTPase-binding:formin homology 3) domain. FHOD1 self-associates via the FH2 domain and binds to F-actin via its N-terminus and also to the cytoplasmic domain of CD21 via its C-terminus. Function of FHOD1 include the assembly of F-actin structures, such as stress fibers, and depends on the Rho-ROCK cascade for its activity. Reports suggest the role of FHOD1 in the coordination of microtubules with actin fibers and also in cell elongation. Endogenous FHOD1 interacts directly with Rac1 in a guanine nucleotide-independent manner and thus activates transcription from the serum response element. Ubiquitous expression of FHOD1 is seen in most of the tissues with abundance in the spleen and a variety of human cell lines.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F6002-67A

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Westendorf, JJ. et al. J. Biol. Chem. 276:46453-46459 (2001). 2. Takeya, R. et al. J. Cell Sci. 116:4567-4575 (2003). 3. Beausoleil, SA. et al. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135 (2004). 4. Gasteier, JE. et al. Exp. Cell. Res. 306:192-202 (2005).

Additional Info

Recognizes human FHOD1. Species Crossreactivity: chimpanzee, canine, equine, mouse, rat, rhesus monkey. Species sequence homology: bovine, opossum.