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Fibrinogen (Coagulation Factor I) (Biotin)

Fibrinogen (Coagulation Factor I) (Biotin)

Cat no: F4199-92H

Supplier: United States Biological
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Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:3000 Optimal dilutions to be determined by the researcher. Biotin/IgG protein molar ratio (B/P): ~6.6 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F4199-92H
Reactivities: Rat
Hosts: Goat
Applications: ELISA
Conjugates: Biotin
Size: 1ml
Form: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Purified by DEAE chromatography.
Additional info: Recognizes rat Fibrinogen. In immunoelectrophoresis and radial immunodiffusion against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of rat plasma.

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