Fibrinogen (Coagulation Factor I)

Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the\nprecursor of fibrin, which is the key protein constituting the network of the blood clot.\nThrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize\nto fibrin which is stabilized by cross-linking. \n\nApplications: \nSuitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:500-1:5000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4199-92G

Size

10mg

Applications

ELISA

Hosts

Goat

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes rat Fibrinogen. In immunoelectrophoresis and radial immunodiffusion against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of rat plasma.