Fibroblast Growth Factor 22 (FGF22)

Fibroblast growth factor 22, a protein which in humans is encoded by the FGF22 gene, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth,morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development.\n\nApplications: \nWestern Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human FGF-22. The detection limit for rhFGF-22 is approximately 10ng/lane under non-reducing and reducing conditions.\nDirect ELISA: This antibody can be used at 0.5-1.0Ug/mL with the appropriate secondary reagents to detect human FGF-22. The detection limit for rhFGF-22 is approximately 1.5 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4212-94A

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human FGF-22 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human FGF-22 in direct ELISAs and Western Blots. In this format, this antibody shows less than 1% cross-reactivity with rhFGF-2, -3, -4, -5, -6, -7, -8b, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -23 and rmFGF-8, rmFGF-15, and rhFGF-acidic.