Fibroblast Growth Factor 6 (FGF6) (Biotin)

Applications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2mg/ml When used in conjunction with the appropriate secondary reagents to detect human FGF-6, the detection limit for rhFGF-6 is ~2ng/lane under non-reducing and reducing conditions.\n\nELISA: The FGF-6 antibody can be used as a detection antibody along with a monoclonal capture antibody (Catalog # F4211-02). Using plates coated with 100ul/well of the capture antibody at 1-2ug/ml with 100ul/well of the detection antibody at 200-400ng/ml. ELISA sample volumes of 100ul with a range of 93.8-6,000pg/ml can be obtained.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4211-02A

Size

50ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Conjugated to Biotin. Reconstitute with sterile Tris-buffered saline, pH 7.3 (20mM Tris base, 150mM NaCl), 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.

P Type

Pab

Purity

Purified by human FGF6 affinity chromatography.

Isotype

IgG

Additional Info

Recognizes recombinant human Fibroblast Growth Factor 6 (FGF6). Crossreactivity: rhFGF-4: ~40%; rhFGF-9: (same/less than) 10%; rhFGF-5 and FGF basic: (same/less than) 5%.