Fibroblast Growth Factor Receptor 3 (FGFR3)

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (MW 17-38kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, and in hematopoiesis as well as in tissue remodelling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150kD proteins consisting of an extracellular domain, a single transmembrane region and a cytoplasmic split tyrosine kinase domain, which is activated following ligand binding and receptor dimerization. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. FGFR3 is widely expressed in many fetal and adult human and animal tissues. The FGFR3 expression profile largely correlates with its tissue specific expression at the mRNA level. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detects a band of ~110 and 120kD using an extract of 293T cells transfected with the human FGFR-3 full length gene. Immunoprecipitation: 1:500 immunoprecipitates FGFR3 from a whole cell lysate of 293T cells transfected with the human FGFR3 full length gene. Immunohistochemistry (Paraffin): 1:1000 stains protease digested, formalin fixed, paraffin embedded human and animal tissue sections by indirect immunoperoxidase staining. The epitope recognized is resistant to routine formalin fixation and paraffin embedding. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.