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Fibroblast Growth Factor Receptor 3 (FGFR3)

Fibroblast Growth Factor Receptor 3 (FGFR3)

Cat no: F4305-18D

Supplier: United States Biological
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Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (Indirect): 1:1000; 293T cells were transfected with FGFR3 plasmide. NBT/BCIP was the substrate. Band at ~110kD. Immunoprecipitation: 0.5-1ug antibody using a whole cell lysate of transfected 293T cells expressing recombinant human FGFR3. Immunohistochemistry (Formalin-fixed paraffin): 1:250 using Tyrpsin-digested sections. Stains human placenta and umbilical cord. Staining is observed in amniotic epithelium, tubules and squamous epithelium. Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysates of transfected 293T (embryonic kidney) cells expressing recombinant human FGFR3. Recommended Secondary Antibodies (WB, IP): I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb Recommended Secondary Antibodies (IHC): I1904-40N: IgG, Fc, X-Adsorbed (Biotin) Pab Gt x Rb I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4305-18D
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Baird, A., et al., Curr. Opin. Neurobiol. 4: 78-86 (1994). 2. Burgess, W.H. & Maciag, T., Annual Rev. Biochem. 58: 575 (1989). 3. Klagsburn, M., Prog. Growth Factor Res. 1: 207 (1989). 4. Givol, D. & Yayon, A., FASEB J. 6: 3362 (1992).
Additional info: Recognizes human Fibroblast Growth Factor Receptor 3 (FGFR3) at ~110kD (doublet). Does not react with human FGFR1 or FGFR2.

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