Fibroblast Growth Factor Receptor beta (Flg, FGFRb, FGFR1)

Currently four FGF receptors have been identi?ed, FGFR-1 (?g), FGFR-2 (bek), FGFR-3, and FGFR-4. FGFR-1 and FGFR-2 exist in multiple forms that represent alternative splicing of their respective genes. The FGF Receptor consists of three immunoglobulin-like domains, a transmembrane region and a cytosolic tyrosine kinase domain which is activated by ligand binding. FGFs play important roles in angiogenesis, wound healing, tumorigenesis and metastasis. The FGF family consists of 7 members, FGF-1 through FGF-7. FGFs 1 and 2 are also known as basic FGF (bFGF) and acidic FGF (aFGF), respectively. It is known that multiple FGFs can bind and activate FGFR-1, FGFR-2, and FGFR-3. \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1ug/ml \nWestern Blot: ~2ug/ml. Detects bands at ~110kD and 120kD.\nImmunoprecipitation: 2-5ug/IP reaction\nImmunohistochemistry (Frozen or Paraffin): 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0/Ag14 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4305-08

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, GP

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

US Biological application reference: Wierzbicki, M. et al., (2013) Nanoscale Research Letters 2013, 8:195 doi:10.1186/1556-276X-8-195. 1. Venkateswaran, S., et al., Hybridoma 11(6): 729 (1992). 2. Panossian, V., et al., Clinical Orthopedic 342: 173-180 (1997).

Additional Info

Recognizes bovine Fibroblast Growth Factor (FGF) Receptor-1 and bovine FGFr. Weakly reacts with FGFR-3. Species Crossreactivity: human, chicken, rat, mouse and guinea pig.