Fibroblast Surface Protein

Cat no: F4213-60

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. Applications: Suitable for use Western Blot, Immunohistochemistry ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Immunohistochemistry: Frozen and paraffin sections Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	F4213-60
Reactivities:	Human
Hosts:	Mouse
Applications:	ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ul
Form:	Supplied as a liquid in PBS., pH 7.4, 1% BSA, 0.1% sodium azide.
P type:	Mab
Isotype:	IgM
Purity:	Ascites
References:	1. Singer, K., et al., J. Invest. Dermatol., 92: 166 (1989). 2. Hogg, N., and Horton, M., Myeloid Antigens. New and previously defined clusters, in: Leukocyte Typing III. McMichael, A., Ed., pp 576-602, Oxford University Press, Oxford (1987). 3. SunderRaj, N., et al., Curr. Eye Res., 3: 637 (1984). 4. Singer, K., et al., Human Immunol., 13: 161 (1985). 5. Abbond, C., et al., Blood, 68: 1196 (1986). 6. Le, P., et al., J. Immunol., 145: 3310 (1990).
Additional info:	Recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. Does not react with vascular smooth muscle cells. May be used for removal of fibroblasts from human cultured cells by inhibition of fibroblast adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.

See Full Product Listings >

Fibroblast Surface Protein

Cat no: F4213-60

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*