Fibroblast Surface Protein

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. \n\nApplications: \nSuitable for use Western Blot, Immunohistochemistry ELISA and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunohistochemistry: Frozen and paraffin sections\nImmunoprecipitation: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4213-60

Size

100ul

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS., pH 7.4, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Singer, K., et al., J. Invest. Dermatol., 92: 166 (1989). 2. Hogg, N., and Horton, M., Myeloid Antigens. New and previously defined clusters, in: Leukocyte Typing III. McMichael, A., Ed., pp 576-602, Oxford University Press, Oxford (1987). 3. SunderRaj, N., et al., Curr. Eye Res., 3: 637 (1984). 4. Singer, K., et al., Human Immunol., 13: 161 (1985). 5. Abbond, C., et al., Blood, 68: 1196 (1986). 6. Le, P., et al., J. Immunol., 145: 3310 (1990).

Additional Info

Recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. Does not react with vascular smooth muscle cells. May be used for removal of fibroblasts from human cultured cells by inhibition of fibroblast adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.\n