Fibroblasts, Epithelial Cells

Cat no: F4213-30B

Supplier: United States Biological

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In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-30B has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes and negative with differentiated chondrocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	F4213-30B
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size:	200ug
Form:	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type:	Mab
Isotype:	IgG2a
Purity:	Purified by Protein G affinity chromatography.
References:	1. Fearns, C. and Dowdle, E. B. (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int. J. Cancer. 50:621-627. 2. Kelynack, K. J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process Nephrol. Dial. Transplant. 15:1766-1772. 3. Van Osch, G.J.V.M. , et al. (2001) Monoclonal antibody 11-Fibrau: A useful marker to characterize chondrocyte differentiation stage. Biochem. Biophys. Res. Commun. 280:806-812.
Additional info:	Recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. Does not recognize mouse or rat.

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Fibroblasts, Epithelial Cells

Cat no: F4213-30B

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