Fibronectin (FN, Fibronectin 1, FN1, Cold-insoluble Globulin, CIG, ED-B, FINC, FNZ, GFND, GFND2, LETS, MSF)

Glycoproteins that have a key role to play in various contact processes within vertebrates such as cell attachment and spreading, cell migration, control of cell morphology, differentiation and oncogenic transformation. It is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. It has a 26 amino acid signal peptide and it undergoes proteolytic processing at its N-terminus to eliminate a 5 amino acid pro-sequence (Ser-Lys-Ser-Lys-Arg). All its biological activities involve its interaction with cells and with extracellular materials like collagen, heparin, fibrin, cell surfaces, bacteria and DNA. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. Plasma FN is secreted by hepatocytes while cellular FN, made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. It maps to 2q34 region of human chromosome.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:50-1:250\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nKidney\n\nCellular Localization: \nBasement membrane and connective tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4215-53W

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. D\\'Ardenne et al. J Clin Pathol 39: 138, 1986. 2. Kirkpatrick et al. J Clin Pathol 37: 639, 1984. 3. Kumar et al. J Clin Pathol 39: 51, 1986. 4. Gutman et al. FEBS Lett 207: 145, 1986. 5. Kornblihtt et al. EMBO J 4: 1755, 1985.

Additional Info

Recognizes human Fibronectin in liver, tonsil, skin and kidney.