FIR (P364) pAb

Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression. The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity. FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators. FIR blocks activator-dependent, but not basal transcription through TFIIH. FIR shares identity with seven in absentia (siah) binding protein 1. FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary.

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SPECIFICATIONS

Catalog Number

BS1895

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 341-390 of Human FIR.

Species

FIR (P364) pAb detects endogenous levels of FIR protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

O94887

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 120 kDa

Alternative Names

60 kDa poly(U)-binding-splicing factor; FBP-interacting repressor; FIR; FLJ31379; fuse-binding protein-interacting repressor; Poly(U)-binding-splicing factor PUF60; poly-U binding splicing factor PUF60; PUF60; PUF60; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; RoBPI; siah binding protein 1; Siah-binding protein 1; Siah-BP1; SIAHBP1