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FKHR, phosphorylated (Ser256) (Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead In Rhabdomyosarcoma, FOXO1A, FOXO1)

FKHR, phosphorylated (Ser256) (Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead In Rhabdomyosarcoma, FOXO1A, FOXO1)

Cat no: F4523-01N

Supplier: United States Biological
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FKHR gene was first identified by virtue of its fusion to the PAX3 gene in the t(2;13) translocation found in the pediatric soft tissue tumor alveolar rhabdomyosarcoma . FKHR gene encodes for a protein containing a DNA-binding motif shared with members of the HNF-3:forkhead family of transcription factors. The protein acts as a potential Akt nuclear target in metabolic and survival pathways. The protein may also play a role in myogenic growth and differentiation. Human FKHR gene is usually localized in the chromosomal region 13q14.1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: Mouse brain tissue Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4523-01N
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Eric, DT. et al. J. Biol. Chem. 274:16741-16746 (1999).
Additional info: Recognizes human FKHR, phosphorylated at Ser256. Species Crossreactivity: mouse and rat.

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