Flag (DYKDDDDK) Epitope Tag

Cat no: F4524-72G

Supplier: United States Biological

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Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:9000-1:450000 Western Blot: 1:2000-1:10000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	F4524-72G
Hosts:	Rabbit
Applications:	ELISA, Western Blot
Size:	100ug
Form:	Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.09% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210. 2. Slootstra, J.W. et al. (1997) Identification of new tag sequences with differential and selective recognition properties for anti-FLAG monoclonal antibodies M1, M2 and M5. Mol. Divers. 2: 156-164.
Additional info:	Recognizes DYKDDDDK TAG.

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Flag (DYKDDDDK) Epitope Tag

Cat no: F4524-72G

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