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Flag (DYKDDDDK) Epitope Tag

Cat no: F4524-72L


Supplier: United States Biological
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The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.2-1.0ug/ml Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4524-72L
Hosts: Chicken/Bird
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
P type: Pab
Isotype: IgY
Purity: Purified by immunoaffinity chromatography.
References: 1. Murshid, Ayesha, Archana Srivastava, Rohit Kumar and John F. Presley, 2006, Characterization of the localization and function of NECAP 1 in neurons. Journal of Neurochemistry 98 (6) , 1746
Additional info: Recognizes Flag Epitope Tag.

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