Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxyl terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 2ug/ml for 2 hours
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry: 1:50 for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:
Angiosarcoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
