FLIP, cellular (cFLIP, FLICE Inhibitory Protein)

cFLIP (cellular FLICE-like inhibitory protein) is a cellular homologue of the viral protein, vFLIP, and is also known as CASH, Casper, CLARP, FLAME, I-FLICE, MRIT and Usurpin (2,3). cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively (3,4). cFLIP can block Fas-mediated apoptosis presumably through the C-terminal region of cFLIPL which has homology with the proteolytic domain of caspase-8 (FLICE). However, cFLIP lacks the active proteinase site required for catalytic activity. Therefore, although it can be recruited to the ligated Fassignalling complex, it cannot deliver a death signal. cFLIP is expressed in the cytosol and it has been reported that cells with high levels of cFLIP relative to FLICE are generally resistant to apoptosis, whereas cells with low levels of cFLIP relative to FLICE are more sensitive to apoptosis (5). cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligimerization of the adapter protein, FADD (5). cFLIP interacts with FADD and caspase-8 through their respective DEDs (2). The regulation and role of cFLIP in mediating signals that promote survival such as NF-kB versus inhibiting cell death by blocking cell death receptor-induced caspase activation is unclear (6).\n\nApplications:\nWestern Blot: 0.5-1ug/ml\nImmunocytochemistry: 5ug/ml\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nHeLa Cell Lysate

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SPECIFICATIONS

Catalog Number

F4550-02

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 6.7-7.0, containing 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Irmler, M., Thome, M., Hahne, M., Schneider, P., Hofmann, K., Steiner, V., Bodmer, J.\nL., Schroter, M., Burns, K., Mattmann, C., Rimoldi, D., French, L. E., Tschopp, J. (1997)\nNature 388:190-195.\n2. Hu WH, Johnson H, Shu HB. (2000) J Biol Chem 275(15): 10838-10844.\n3. Thomas RK, Kallenborn A, Wickenhauser C, Schultze JL, Draube A, Vockerodt M, Re\nD, Diehl V, Wolf J. (2002) Am J Pathol 160(4): 1521-1528.\n4. Hennino A, Berard M, Casamayor-Palleja M, Krammer PH, Defrance T. (2000) J\nImmunol 165(6): 3023-3030.\n5. Panka DJ, Mano T, Suhara T, Walsh K, Mier JW. (2001) J Biol Chem 276(10): 6893-\n6896.\n6. Rathmell, J. C., Thompson, C. B. (2002) Cell 109: S97-S107

Additional Info

This immunoaffinity purified antibody detects 28-58kD proteins, corresponding to the apparent molecular mass of all c-FLIP variants on SDS-PAGE immunoblots, in human, mouse and rat cell lysates and tissue extracts.