FLIP gamma/delta, CT (FLIP gamma, FLIP delta)

FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. \n\nFLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. \n\nFLIP is widely expressed in human tissues and a diverse range of cell lines. \n\nApplications:\nSuitable for use in Immunohistochemistry (frozen) and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 2ug/ml\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4550-50

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Thome M., et al (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 386:517-521. 2. Irmler M., et al (1997) Inhibition of death receptor signals by cellular FLIP. Nature 388:190-195. 3. Shu H.B., et al (1997) Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 6:751-763. 4. Hu S., et al (1997) I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 272:17255-17257.

Additional Info

Recognizes human FLIP gamma/DELTA, CT. Species Crossreactivity: mouse.