FLIP, NT (CASP8 and FADD-like Apoptosis Regulator, Caspase Homolog, CASH, Caspase-eight-related Protein, Casper, Caspase-like Apoptosis Regulatory Protein, CLARP, Cellular FLICE-like Inhibitory Protein, c-FLIP, FADD-like Antiapoptotic Molecule 1, FLAME-1,

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, beta, gamma, and delta respectively.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4550-05E

Size

50ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Thome, M. et al. Nature 386, 517 (1997). 2. Irmler, M. et al. Nature 388, 190 (1997). 3. Shu, HB. et al Immunity 6, 751 (1997). 4. Hu, S. et al. J. Biol. Chem. 272, 17255 (1997). 5. Srinivasula, SM. et al J. Biol. Chem. 272, 18542 (1997). 6. Goltsev, YV. et al. J. Biol. Chem. 272, 19641 (1997). 7. Inohara N. Proc. Natl. Acad. Sci. USA 94, 10717 (1997). 8. Wallach D. et al. Nature 388, 123 (1997).

Additional Info

Recognizes human FLIP. Species Crossreactivity: mouse and rat.