Flt1 (Fms-like Tyrosine Kinase 1, Vascular Endothelial Growth Factor Receptor 1, VEGF R1)

Human Flt-1 is a type I transmembrane receptor for the angiogenic cytokine, VEGF. Alternate splicings of human VEGF R1 mRNA encode soluble, secreted isoforms that can function as inhibitors of VEGF. One, designated sFLT1-14 or sFLT1-e15a, encodes a 733aa, 95-135kD form that is produced by non-endothelial cells, notably by degenerative syncytiotrophoblasts within syncytial knots in the placenta. This form appears to be unique to primates and is produced in excess during preeclampsia of pregnancy. The peptide used as an immunogen is not present within either the full-length or alternate secreted forms of the protein.\n\nApplications:\nSuitable for use in Western Blot and Direct ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 2ug/ml using human serum (preeclampsia)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4599-03G

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human Flt1. In Direct ELISA and Western Blot there is no crossreactivity with recombinant mouse VEGF R1, recombinant human VEGFR1, VEGF R2, or recombinant human VEGF R3.