FMO3 (Dimethylaniline oxidase 3, FMO II, FMO form 2, Hepatic flavin-containing monooxygenase 3)

FMO3(Flavin-containing Monooxygenase 3) is an enzyme that in humans is encoded by \nthe FMO3 gene. The mammalian flavin-containing monooxygenases (FMO) represent a multigene \nfamily whose gene products are localized in the endoplasmic reticulum of many \ntissues. The FMO3 gene contains 1 noncoding and 8 coding exons. The FMO3 gene is mapped on \n1q24.3. Using quantitative RNase protection assays, FMO3 is present in low abundance in fetal liver \nand lung and in adult kidney and lung, and in much greater abundance in adult liver. By Western blot \nanalysis of human liver microsomal samples ranging from 8 weeks gestation to 18 years of age, FMO1 \nis the major fetal isoform and FMO3 is the major adult isoform. FMO3 was expressed at intermediate \nlevels until 11 years of age when a gender-independent increase in FMO3 expression was observed \nduring puberty. Sufferers of trimethylaminuria may display a reduced ability to metabolize substrates \nfor FMO3 such as nicotine. FMO3 metabolizes a number of drugs, including amphetamine, clozapine, \ndeprenyl, metamphetamine, tamoxifen, ethionamide, thiacetazone, and sulindac sulfide.\n\nUniProt Number:\nP31513\n\nGene ID:\nFMO3\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144544

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Akerman, B. R., Chow, L., Forrest, S., Youil, R., Cashman, J., Treacy, E. P. Mutations in the \nflavin-containing monoxygenase (sic) form 3 (FMO3) gene cause trimethylaminuria, fish odour \nsyndrome. (Abstract) Am. J. Hum. Genet. 61 (suppl.): A53 only, 1997.\n2. Cashman, J. R., Zhang, J. Interindividual differences of human flavin-containing monooxygenase 3: \ngenetic polymorphisms and functional variation. Drug Metab. Dispos. 30: 1043-1052, 2002.\n3. Dolphin, C. T., Janmohamed, A., Smith, R. L., Shephard, E. A., Phillips, I. R. Missense mutation in \nflavin-containing mono-oxygenase 3 gene, FMO3, underlies fish-odour syndrome. Nature Genet. 17: \n491-494, 1997.

Additional Info

Recognizes human, mouse and rat FMO3. No crossreactivity with other proteins.