Folate Binding Protein (FBP, Folate Receptor 1 Adult, Folate Receptor 1 Precursor, FOLR1, FRalpha, KB Cells FBP, MOV18 Ovarian Cancer Associated Antigen, Ovarian Tumor Associated Antigen) (HRP)

Folate Binding Protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.\n\nApplications: \nSuitable for use in ELISA and Western Blot.Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Predicted molecular weight 31kD.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F5750-15A

Size

2mg

Applications

ELISA, WB

Hosts

Goat

Form

Supplied as a liquid in PBS, 10mg/ml Polyethylene glycol (PEG 8000). Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified

Isotype

IgG

References

US Biological application reference: Achanta, K. et al., (2007) J. Dairy Sci. 90:90-98.

Additional Info

This antibody targets both purified and partially purified Folate Binding Protein from bovine milk. Species Crossreactivity: Crossreacts with bovine.Not yet tested in other species.