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Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

Cat no: F5750-15B

Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

Folate receptor 1 (FOLR1; also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family. FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The human FOLR1 preproprecursor is 257aa in length. It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234). There are two forms of FOLR1. One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226). There is one potential alternate splice form that shows a five aa substitution for aa 121-205. Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1. \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume (same/less than)200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer.\nDirect ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.\nWestern Blot: 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F5750-15B

Size

100ug

Applications

ELISA, FC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human FOLR1. Shows < 5% crossreactivity with recombinant human FOLR2 and FOLR3 in direct ELISAs.

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