Folate Transporter (FOLT, RFC, Reduced Folate Carrier)

A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc.\n\nFolate Transporter/ FOLT/ RFC, a 512aa each protein in mouse and rat, 591aa in human (chrm 21q22.3, gene SLC19A1) transports folate compounds into mammalian cells via receptor mediated or carrier mediated mechanisms, as such maintains the intracellular concentration of folate. It shows 65% identity to mouse and hamster folate transporters.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. F5753-40C Control peptide can be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1-10ug/ml (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F5753-40B

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Prasad P. D (1995) Biochem Biophys Res commun, 206 (2) 681-687. 2. Wong S. C (1995) JBC 270 (29), 17468-75. 3. Moscow J. A (1995) Cancer Res 55 (17), 3790-94.

Additional Info

Recognizes rat RFC.