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Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

Cat no: F5800-11

Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

Applications:\nSuitable for use in ELISA, Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:2000-5000\nImmunohistochemistry: 1:500-1000. Folic Acid-immunoreactive fibers located in the ventro-lateral part of the pulvinar nucleus of monkey brain. Also, immunoreactive fibers containing Folic acid in the dorsal mesencephalic region of the monkey brain. Fibers located between the ventricul and the lateral geniculate nucleus of the thalamus. \nOptimal dilutions to be determined by the researcher.\n\nSpecificity:\nUsing a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :\n\nCompound Cross-reactivity ratio (a)\n\nFolic acid-BSA 1\nFolinic acid-BSA 1/>900\nTetrahydrofolic acid-BSA 1/>2,000\nMethotrexate-BSA 1/>50,000\nRiboflavin-BSA 1/>50,000\nBSA 1/>50,000\n\n(a): Folic acid-BSA concentration/unconjugated or conjugated compound concentration at half displacement.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F5800-11

Size

100ug

Applications

ELISA, IHC

Hosts

Rat

Form

Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes protein-conjugated Folic Acid (Vitamin B9). See additional crossreactivity data.

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