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Follistatin 315 (CT) (FSTL1, FS315)

Cat no: F9090-02A

Follistatin 315 (CT) (FSTL1, FS315)

Follistatin (FST) is a single-chain glycosylated protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion. Alternative splicing of FST mRNA generates the two isoforms FS315 and FS288, the latter of which is the main cell-surface form and binds with high affinity to surface heparan sulphate proteoglycans, whilst FS315 binds with only low-affinity, and is considered to be the main circulating form of follistatin. As well as activin, FST interacts with other growth factors such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin, and growth differentiation factor 9 (GDF-9), playing a key role in cellular differentiation/proliferation, embryonic development and tissue repair. \n\nApplications:\nSuitable for use in ELISA, Western Blot, and Immunohistochemistry (paraffin). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTestis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9090-02A

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. McPherson, S.J. et al. (1999) Expression of activin A and follistatin core proteins by human prostate tumor cell lines. Endocrinology. 140: 5303-5309.

Additional Info

Recognizes an epitope within the C-terminal region of human follistatin 315 (FS315). Does not cross-react with FS288.

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