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Formin Binding Protein 1-like, NT (C1orf39, FLJ20275, TOCA1, Toca-1, Transducer of Cdc42-dependent actin assembly protein 1)

Formin Binding Protein 1-like, NT (C1orf39, FLJ20275, TOCA1, Toca-1, Transducer of Cdc42-dependent actin assembly protein 1)

Cat no: F6002-63

Supplier: United States Biological
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Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N- WASP and Cdc42 and is essential for Cdc42- and PIP2- induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex (1). Decreased expression of TOCA- 1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation (2). It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain (2). Multiple isoforms of TOCA-1 are known to exist. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F6002-63
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell 2004; 118:203-16. 2. Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem. 2006; 281:29042-43.
Additional info: Recognizes human TOCA-1. Species crossreactivity: Mouse and rat.

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