Fos, phosphorylated (Thr232) (c-Fos)

c-Fos is a ~60kD immediate early gene product and a member of AP-1 family. c-Fos dimerizes with c-Jun to form the transcriptionally active complex AP-1, and functions in regulating cell proliferation and differentiation. c-Fos expression and activation are induced in many tumors including breast cancer), and in response to a variety of stimuli (including growth factors, cytokines and stress). ERK1&2 mediate growth factor-induced c-Fos induction and transcripional activation by directly phosphorylating the carboxyl terminal domain of c-Fos. c-Fos is phosphorylated by Ras on threonine 232, the homologue of serine 73 of c-Jun, which results in augmenting its transcriptional activity.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6040-20

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+) pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Lu, Y., et al. (2004) Overexpression of ErbB2 receptor inhibits IGF-I-induced Shc-MAPK signaling pathway in breast cancer cells. Biochem. Biophys. Res. Commun. 313(3):709-715. 2. Monje, P., et al. (2003) Phosphorylation of the carboxyl-terminal transactivation domain of c-Fos by extracellular signal-regulated kinase mediates the transcriptional activation of AP-1 and cellular transformation induced by platelet-derived growth factor. Mol. Cell. Biol. 23(19):7030-7043. 3. Mikula, M., et al. (2003) The proto-oncoprotein c-Fos negatively regulates hepatocellular tumorigenesis. Oncogene 22(43):6725-6738.\n4. Wang, L.H., et al. (2003) The cis decoy against the estrogen response element suppresses breast cancer cells via target disrupting c-fos not mitogen-activated protein kinase activity. Cancer Res. 63(9):2046-2051. 5. Deng, T. and M. Karin (1994) c-Fos transcriptional activity stimulated by H-Ras-activated protein kinase distinct from JNK and ERK. Nature 371(6493):171-175.

Additional Info

Recognizes human c-Fos when phosphorylated at Thr232. Species homology: mouse and rat c-Fos (100%).