Fos Related Antigen 2 (FRA2)

Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, Fra 1, and Fra 2 (Fos Related Antigen).\n\nFos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.\n\nCellular Localization: Nuclear\n\nApplications: \nSuitable for use in GS, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6045-07

Size

200ul

Applications

IHC, WB

Hosts

Rabbit

Form

Whole Serum

P Type

Pab

Purity

Whole antisera

Isotype

IgG

References

Huguier S et al.> Transcription factor ATF2 cooperates with v-Jun to promote growth factor-independent proliferation in vitro and tumor formation in vivo. Mol Cell Biol 18:7020-9 (1998). <PUBMED:9819389>van Dam H et al. Autocrine growth and anchorage independence: two complementing Jun-controlled genetic programs of cellular transformation. Genes Dev 12:1227-39 (1998). <PUBMED:9553051>Vandel L et al. Stepwise transformation of rat embryo fibroblasts: c-Jun, JunB, or JunD can cooperate with Ras for focus formation, but a c-Jun-containing heterodimer is required for immortalization. Mol Cell Biol 16:1881-8 (1996). <PUBMED:8628254>Jurdic P et al. Tumor induction by v-Jun homodimers in chickens. Oncogene 10:495-507 (1995). <PUBMED:7845674>

Additional Info

Reacts specifically with 32-45kD protein derived from a transfected CEF cell line. Species Crossreactivity: Crossreacts with Birds.