FosB

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates (1,2) and an avian nephroblastoma virus (3). The cellular homolog, c-Fos, encodes a 62kD nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers, which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family (4,5). Murine FosB encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements (6). Functional homologs of c-Fos and FosB include Fra-1 and Fra-2 genes (7). \n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 2ug/ml; Band at 46kD.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6042-01A

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Finkel, M.P., et al. Virology 135: 218-228 (1984). 2. Nishizawa, M., et al. J. Virol. 61: 3733-3740 (1987). 3. Sambucetti, L.C. and Curran, T. Science 234: 1417-1419 (1986). 4. Renz, M., et al. Nucl. Acids Res. 15: 277-292 (1987). 5. Castellazzi, M., et al. Proc. Natl. Acad. Sci. USA 88:8890-8894 (1991). 6. Nishina, H., et al. Proc. Natl. Acad. Sci. USA 87: 3619-3623 (1990). 7. Zerial, M., et al. EMBO J. 8: 805-813 (1989). 8. Sabatakos G, et al. Nat Med., 6(9):985-90. (2000). \n

Additional Info

Recognizes human FosB.