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FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF3A, MGC33105, TCF3A)

Cat no: F9045-02A

FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF3A, MGC33105, TCF3A)

FOXA1 (HNF3A, Hepatocyte nuclear factor 3- alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor alpha, in each of the tumor type respectively (Lupien, 2008). In the case of ERa, FOXA1 has gained notice because of its interactions with the cis- regulatory regions in heterochromatin and because it enhances the interaction of ERa with chromatin (Badve, 2007).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nImmunohistochemistry (Paraffin): 1:2000\nImmunocytochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9045-02A

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Lupien, M. et al. (2008) Cell. March 21; 132(6): 958

Additional Info

Recognizes human FOXA1 (HNF3a) at ~49kD. Species Crossreactivity: mouse.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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