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FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis)

FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis)

Cat no: F9046-10C

Supplier: United States Biological
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Embryonic stem cells require the forkhead transcriptional regulator FoxD3 for survival. Following gastrulation, FoxD3 generally gets downregulated, except in the neural crest. A variety of growth factors induce FoxD3 expression, including FGF8 and SNAIL, maintaining the effected cells in an undifferentiated state. Thus defects in FoxD3 induction may cause premature differentiation and/or migration-asociated birth defects. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (HUVEC cells): 1:500-1:1000 Immunohistochemistry (Paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F9046-10C
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes endogenous levels of human FOXD3. Species Crossreactivity: mouse.

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