FOXM1 (Forkhead Box Protein M1, Forkhead-related Protein FKHL16, Hepatocyte Nuclear Factor 3 Forkhead Homolog 11, HFH11, HFH-11, HNF-3, HNF-3/fork-head Homolog 11, INS-1, M-phase Phosphoprotein 2, MPP2, MPP-2, MPM-2 Reactive Phosphoprotein 2, MPHOSPH2, PI

Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331 Ser704), Cyclin/ Cdk (Thr600, Thr611, Thr672), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-7). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (8). Recent studies have shown that FoxM1 expression is negatively \nregulated by p53 (9,10). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (10,11). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (12). \n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:50\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9048-25D

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Wang, I.C. et al. (2005) Mol Cell Biol 25, 10875-94. (2) Leung, T.W. et al. (2001) FEBS Lett 507, 59-66. (3) Wang, X. et al. (2002) Proc Natl Acad Sci USA 99, 16881-6. (4) Ma, R.Y. et al. (2005) J Cell Sci 118, 795-806. (5) Laoukili, J. et al. (2008) Mol Cell Biol 28, 3076-87. (6) Fu, Z. et al. (2008) Nat Cell Biol 10, 1076-82. (7) Tan, Y. et al. (2007) Mol Cell Biol 27, 1007-16. (8) Ye, H. et al. (1997) Mol Cell Biol 17, 1626-41. (9) Barsotti, A.M. and Prives, C. (2009) Oncogene 28, 4295-305. (10) Pandit, B. et al. (2009) Cell Cycle 8, 3425-7. (11) Pilarsky, C. et al. (2004) Neoplasia 6, 744-50. (12) Gartel, A.L. (2008) Expert Opin Ther Targets 12, 663-5.

Additional Info

Recognizes endogenous levels of total FoxM1.