GCSm-(gamma) (V91) pAb

Gamma-glutamylcysteine synthetase ((gamma)-GCS) is the rate limiting enzyme for glutathione (L-(gamma)-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. (gamma)-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines. The human (gamma)-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human (gamma)-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of (gamma)-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

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SPECIFICATIONS

Catalog Number

BS2927

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 50-100 of Human GCSm-(gamma).

Species

GCSm-(gamma) (V91) pAb detects endogenous levels of GCSm-(gamma) protein.

Dilutions

WB: 1:500~1:1000\r\nIHC: 1:50~1:200

Swiss Prot

P48507

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 30 kDa

Alternative Names

Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit; GLCLR; GCLM