GFP-Tag Mouse mAb

Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

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SPECIFICATIONS

Catalog Number

MB2005

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Amo/Prot, Bac/Arc, Bov, Worm, Can, Chem, Ch/Bird, Dnk, Dro/Arth, ETag, Fel, Goat, GP, Ham, Horse, Moll, NHP, Plant, Prc, Rept, Rabbit

Form

liquid

Purity

Affinity-chromatography

Antigen

Synthetic peptide GFP-Tag.

Species

GFP-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.

Dilutions

WB: 1:5000~1:10000\nIHC: 1:200\nIF: 1:200

Swiss Prot

N/A